Remediation engine

Engineer microbes that break down a pollutant

Enter a contaminant — GeneFence designs the enzymes, picks host microbes, and returns a ranked, safety‑gated shortlist of cleanup strategies to test.

Pollutant

Try a tour of the states:

You bring the edit; GeneFence pressure-tests it. Give the target microbe, the CRISPR/DART edit, and the resident microbiome below — we run a calibrated, abstaining 3-axis pre-screen: specific · survivable · contained. It never assumes your design is right.

Target microbe — the resident member you intend to edit

Editing enzyme — sets the PAM, spacer, seed & mechanism Target sequence — the protospacer (your guide), 5'→3' · PAM excluded · RNA or DNA · scaffold fixed by the nuclease

Insert — optional · the inserted gene DNA (donor / payload)

SURVIVABILITY INPUTS — both auto-derived from your guide + the target genome on Analyze; set them only to override

Disrupted locus — optional override · auto-derived by matching your guide to the target's genes (the CDS it cuts) Host DNA-repair (recA / SOS) — optional override · auto-detected by scanning the target genome for recA

Resident microbiome — neighbours A1 scans for collateral; add by name, or upload FASTAs below

No data handy?

Ecosystemthe community candidate hosts are drawn from — and that escape risk is scored against

First run after idle can take ~30s (serverless cold start). Subsequent identical runs are cached & instant.

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Methodology

How GeneFence works

Click any step for its method & references

Validation cascade

↺ re-ranks the shortlist

Discovery pipeline

Design lanes

Ranked shortlistsafety-gated strategies

Architecture

The stack behind GeneFence

Click any service for its role, scope & what it’s built with

Browserclient

Edge

API

Compute

GPU · scale-to-zero

Data

Ops

About

About GeneFence

Making it a little easier — and safer — to explore cleaning up pollution with engineered microbes.

A small project with one honest goal.

Make it a little easier — and safer — to explore cleaning up pollution with engineered microbes.

GeneFence started with a student curious about using engineered bacteria to break down pollutants, and bothered that most tools skip the obvious risk: that the microbes, or their genes, escape. So this one puts safety in the same answer. The science came from her; the software from her dad.

Lelani Laruelle

Founder

Incoming UC Berkeley student in Molecular & Cell Biology, and a two-time California High Jump Champion. Interested in GMO research for bioremediation — done responsibly.

Frederic Laruelle

Build

Lelani's dad. Turned the idea into working, hosted software.

Saved runs

History

Past analyses, saved in this browser. Re-open any run instantly.

Reference

Catalog

What the friendly names actually mean — the pollutants we resolve to structures, the real genomes behind each ecosystem’s escape community, and the host microbes you can engineer.